TMEM106B Knockout cell line (A549)

TMEM106B Knockout cell line (A549) Order

Catalog#

YKO-XN75157

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

TMEM106B Knockout cell line (A549)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

TMEM106B

Gene ID

54664

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% F12K+10% FBS

Target region

Knockout region: Exon 2 (based on transcript TMEM106B-202)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM106B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM106B gene knockout TMEM106B Knockout cell line (A549) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

TMEM106B Knockout cell line (A549)

Catalog#

YKO-XN75157

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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TMEM106B Knockout cell line (A549)

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